rs12976445

This study identified SLC24A2 as a target gene of oar-miR-377 microRNA in sheep, and discovered a novel SNP (276T>C) in the upstream flanking region of oar-miR-377 that is extremely significantly associated with wool fiber diameter variation (P<0.01) in Chinese Merino sheep. Functional studies using dual-luciferase reporter assays and Western blotting confirmed that oar-miR-377 regulates SLC24A2 expression and promotes secondary hair follicle development.

A meta-analysis investigating the association of B7-H4 gene rs10754339 (A/G) and miR-125a gene rs12976445 (T/C) with cancer susceptibility across multiple cancer types. The study included original case-control data from 1,490 Chinese cancer patients (liver, lung, and gastric cancers) and 800 controls, plus meta-analysis of 8 studies for rs10754339 and 8 studies for rs12976445. Results showed rs10754339 A-allele was protective against overall cancer (OR 0.78, 95% CI 0.66-0.93) and breast cancer specifically (OR 0.73, 95% CI 0.64-0.85) in Chinese populations, while rs12976445 T-allele increased cancer risk overall (OR 1.28, 95% CI 1.13-1.45 in Chinese population).

This study examined the rs531564 SNP in microRNA-124 (miR-124) and its role in anal abscess development in anal fissure patients. Using luciferase assays and clinical samples from 108 AF patients (40 with anal abscess, 68 without), the authors found that miR-124 directly targets IL-6R mRNA and downregulates its expression. Patients carrying the TC/TT genotype of rs531564 showed reduced miR-124 expression and elevated inflammatory markers (TNF-α, IFN-γ, IL-4), with an increased incidence of anal abscess formation. The rs12976445 SNP in miR-125a showed no significant association with anal abscess risk.

A case-control association study of 50 Saudi women with recurrent pregnancy loss (RPL) and 50 healthy controls examined six microRNA gene polymorphisms. Significant associations were found for miR-146a C>G (rs2910164, OR=2.29, 95% CI: 1.02-5.18, p=0.046) and miR-149 T>C (rs2292832, OR=2.67, 95% CI: 1.08-6.61, p=0.034) in heterozygous genotypes, with higher frequency in RPL patients compared to controls. Other polymorphisms (miR-10, miR-125, miR-323b, miR-499) showed no significant association with RPL.